The Cestello Annunciation, is a painting in tempera on panel made in 1489 by Sandro Botticelli. It was painted for the patron Benedetto di Ser Giovanni Guardi to adorn the church of the Florentine monastery of Cestello, which is now known as Santa Maria Maddalena de'Pazzi.
The subject of the painting is the Annunciation, in which the archangel Gabriel visits the Virgin Mary to 'announce' to her (hence 'Annuciation') that she has been chosen by God to bear the Christ child should she accept this invitation. Her 'fiat' (Let it be done to me) is her response. Underneath the painting on its original frame are words in Latin from St. Luke's Gospel 1:35 "The Holy Ghost shall come upon thee, and the power of the Highest shall overshadow thee."