The Sacrifice of Isaac is a fresco painting by Giovanni Battista Tiepolo (1696–1770), created in 1726. Measuring 500 × 400 cm, it depicts the biblical scene from Genesis 22. This painting is housed at Palazzo Patriarcale, Piazza del Patriarcato, Udine.
An angel keeps Abraham from sacrificing his son Issac, as God had commanded him to do as a test of his faithfulness. The loose paint handling and daring composition of this painting are very similar to works by his father Giovanni Battista Tiepolo. It was probably painted when both artists worked together on the decoration of the Residenz at Würzburg.